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KMID : 0861020060210040143
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Korea Journal of Herbology
2006 Volume.21 No. 4 p.143 ~ p.148
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Effect of Antioxidant of Citri Reticulatae Pericarpium on Cytotoxicity of Oxygen Free Radicals in Cultured NIH3T3 Fibroblast
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Oh Yong-Leul
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Abstract
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Objectives : It is demonstrated that oxygen free radicals have cytotoxic effect on NIH3T3 fibroblast cells. Recently, many of herb extracts have an effect of antioxidant in oxygen free radical-induced cytotoxicity. But, the toxic mechanism of oxygen free radical is left unknown. The purpose of this study was to examine the cytotoxicity of hydrogen peroxide (H_2O_2) and antioxidant effect of Citri reticulatae pericarpium (CRP) on NIH3T3 fibroblasts.
Methods : The cytotoxicy was measured by cell viability by XTT assay in NIH3T3 fibroblasts. XTT assay is regarded as a very sensitive screening method for the determination of the cell viability on various chemicals.
Results : In this study, H2O2 decreased cell viability according to the dose- and time dependent manners after NIH3T3 fibroblasts were treated with various concentrations of H2O2 for 4 hours. And also, CRP showed the effect of antioxidant on H_2O_2-induced cytotoxicity in cultured NIH3T3 fibroblasts.
Conclusion : These results suggest that H_2O_2 has highly cytotoxic effect on cultured NIH3T3 fibroblasts by the decrease of cell viavility, and the herb extract such as CRP was showed the effect of antioxidant on H_2O_2-induced cytotoxicity in these cultures.
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KEYWORD
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Oxygen free radicals, Hydrogen peroxide, Citri reticulatae pericarpium
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